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StarD4 is a member of the StarD4 subfamily of START domain proteins with a characteristic lipid binding pocket specific for cholesterol. The objective of this study was to define StarD4 subcellular localization, regulation, and function. Immunobloting showed that StarD4 is highly expressed in the mouse fibroblast cell line 3T3-L1, in human THP-1 macrophages, Kupffer cells (liver macrophages), and hepatocytes.
In 3T3-L1 cells and THP-1 macrophages, StarD4 protein appeared localized to the cytoplasm and the endoplasmic reticulum (ER). More specifically, in THP-1 macrophages StarD4 co-localized to areas of the ER enriched in Acyl-CoA:cholesterol acyltransferase-1 (ACAT-1), and was closely associated with budding lipid droplets. The addition of purified StarD4 recombinant protein to an in vitro assay increased ACAT activity 2-fold, indicating that StarD4 serves as a rate-limiting step in cholesteryl ester formation by delivering cholesterol to ACAT-1-enriched ER.
In addition, StarD4 protein was found to be highly regulated and to redistribute in response to sterol levels. In summary, these observations, together with our previous findings demonstrating the ability of increased StarD4 expression to increase bile acid synthesis and cholesteryl ester formation, provide strong evidence for StarD4 as a highly regulated, non-vesicular, directional, intracellular transporter of cholesterol which plays a key role in the maintenance of intracellular cholesterol homeostasis. Introduction Cholesterol is an important structural component of mammalian cell membranes, and serves as a precursor to bile acids (in the liver), steroid hormones (in the adrenal, testis and ovaries), and vitamin D. Homeostasis of cholesterol within the body is maintained through the coordinate regulation of its cellular mediated uptake, transport/trafficking, sorting, biosynthesis, storage (i.e. Esterification), secretion, and catabolism to bile acids []. In recent years, a number of specialized non-vesicular lipid transporters that are part of the steroidogenic acute regulatory related lipid transfer (START) domain superfamily of proteins have been shown to be involved in the trafficking of cholesterol and other lipids between intracellular membranes [–]. All proteins with a START domain contain a similar binding pocket, where modifications determine ligand-binding specificity and function [].
START domains are 200–210 amino acid motifs, which appear in a wide range of proteins, and have been implicated in several cellular functions including lipid transport and metabolism, signal transduction, and transcriptional regulation [,, ]. More specifically, a StarD4 subfamily of START domain proteins, that includes StarD4, StarD5 and StarD6, contains 205–233 amino acid residues and shares 26–32% identity [].